健康植物和病毒感染植物中双链RNA的免疫化学检测以及通过与标记的互补DNA杂交对病毒双链RNA进行特异性检测。

Immunochemical detection of ds-RNA in healthy and virus-infected plants and specific detection of viral ds-RNA by hybridization to labelled complementary DNA.

作者信息

Gould A R, Francki R I

出版信息

J Virol Methods. 1981 Apr;2(5):277-86. doi: 10.1016/0166-0934(81)90026-4.

Abstract

Double-stranded (ds) RNA was detected in nucleic acid preparations from virus-free leaf tissues of several plant species by radial double-diffusion tests with antiserum raised against polyinosinic : polycytidylic acid [poly(I) : poly(C)]. No significant differences in the concentrations of ds-RNA were detected by such tests in virus-free French bean leaves and those infected with tobacco mosaic virus or tobacco ringspot virus. However, virus-specific ds-RNAs as well as genomic RNAs of both viruses were readily detected and estimated quantitatively by hybridization to 3H-labelled complementary DNA probes. It is concluded that it is not feasible to screen plant material infected with single-stranded RNA viruses by immunochemical tests for virus-associated ds-RNAs.

摘要

通过用针对聚肌苷酸

聚胞苷酸[poly(I) : poly(C)]产生的抗血清进行放射状双向扩散试验，在几种植物物种的无病毒叶片组织的核酸制剂中检测到了双链（ds）RNA。通过此类试验，在无病毒的菜豆叶片以及感染烟草花叶病毒或烟草环斑病毒的叶片中，未检测到ds-RNA浓度有显著差异。然而，通过与3H标记的互补DNA探针杂交，可以很容易地检测到并定量估计两种病毒的病毒特异性ds-RNA以及基因组RNA。得出的结论是，通过针对病毒相关ds-RNA的免疫化学试验来筛选感染单链RNA病毒的植物材料是不可行的。