Rybal'chenko V K, Karamushka V I, Gruzina T G, Pogrebnoĭ P V, Kulikova N V
Ukr Biokhim Zh (1978). 1983 Sep-Oct;55(5):552-6.
A method is described for reconstruction of certain sarcolemma characteristics of smooth muscle cells in the small intestine of a rabbit on the planar lipid membranes (PLM). The method is based on the use of fusogenic properties of certain lipid preparations. The ultrasound dispergates of azolectin and egg lecithin in combined incubation with sarcolemma vesicles of smooth-muscle cells promote a 1.4-1.8-fold increase of the total ATPase activity of the sarcolemma. Cholesterol, dipalmithoil lecithin, total brain phospholipids, inhibit the ATPase activity. Sarcolemma vesicles preincubated with azolectin lyposomes in the ratio which induces maximum ATPase activation (sarcolemma protein: azolectin-1:6) interact intensively with PLM from azolectin. PLM modified in such a way is channel-conductive, sensitive to tetraethylammonium and sign of the applied voltage.
描述了一种在平面脂质膜（PLM）上重建兔小肠平滑肌细胞某些肌膜特性的方法。该方法基于某些脂质制剂的融合特性。偶氮卵磷脂和卵磷脂的超声分散液与平滑肌细胞的肌膜囊泡联合孵育可使肌膜的总ATP酶活性提高1.4 - 1.8倍。胆固醇、二棕榈酰卵磷脂、全脑磷脂抑制ATP酶活性。以诱导最大ATP酶激活的比例（肌膜蛋白：偶氮卵磷脂 - 1:6）与偶氮卵磷脂脂质体预孵育的肌膜囊泡与来自偶氮卵磷脂的PLM强烈相互作用。以这种方式修饰的PLM具有通道传导性，对四乙铵和施加电压的符号敏感。
