Raska K, Raskova J, Shea S M, Frankel R M, Wood R H, Lifter J, Ghobrial I, Eisinger R P, Homer L
Am J Med. 1983 Nov;75(5):734-40. doi: 10.1016/0002-9343(83)90401-1.
The T lymphocyte population was studied by immunofluorescent staining with monoclonal antibodies and laser flow cytometry in the blood of 50 patients with end-stage renal disease undergoing long-term maintenance intermittent hemodialysis. The absolute number of T cells was lower in patients receiving dialysis for more than one year (p less than 0.001), as was the absolute count of helper T cells (p less than 0.005). In patients under 30 years of age, the absolute number of helper T cells was markedly reduced, whereas the number of suppressor/cytotoxic T lymphocytes was not changed. In patients between the ages of 30 and 60 years, both helper and suppressor cells were significantly reduced. In patients over 60 years of age, only the number of helper T cells was reduced. The in vitro response of patients' lymphocytes was reduced both in the mixed lymphocyte reaction (p less than 0.01) and after phytohemagglutinin stimulation (p less than 0.001). Natural killer cytotoxicity of patients' peripheral blood mononuclear cells, however, was unaffected.
采用单克隆抗体免疫荧光染色及激光流式细胞术，对50例接受长期维持性间歇性血液透析的终末期肾病患者的血液中的T淋巴细胞群体进行了研究。透析超过一年的患者T细胞绝对数较低（p<0.001），辅助性T细胞绝对计数亦如此（p<0.005）。在30岁以下的患者中，辅助性T细胞绝对数显著降低，而抑制性/细胞毒性T淋巴细胞数量未改变。在30至60岁的患者中，辅助性T细胞和抑制性T细胞均显著减少。在60岁以上的患者中，仅辅助性T细胞数量减少。患者淋巴细胞的体外反应在混合淋巴细胞反应中降低（p<0.01），在植物血凝素刺激后也降低（p<0.001）。然而，患者外周血单个核细胞的自然杀伤细胞毒性未受影响。
