Kojima A, Thomas W R, Mottram P L, Miller J F
Int Arch Allergy Appl Immunol. 1983;72(4):299-302. doi: 10.1159/000234887.
Long-term cultures of functional T cells were grown from mice injected with azobenzene arsonate (ABA) coupled spleen cells or mice painted with the diazonium salt of p-phenylarsonate. All cell lines produced delayed hypersensitivity to ABA. When tested for helper function in an antibody-forming cell assay only one line, AA3, helped plaque-forming cell formation. It was able to help anti-FLU response of FLU-HGG primed B cells to ABA-KLH-FLU. The help required linkage of the ABA and FLU determinants because cultures stimulated with a mixture of ABA-KLH and TNP-KLH-FLU did not show increased PFC. These experiments demonstrate hapten-specific help in a system which eliminates involvement of passively absorbed antibody in hapten recognition.
从注射了偶氮苯胂酸盐（ABA）偶联脾细胞的小鼠或用对苯胂酸重氮盐涂抹的小鼠中培养出功能性T细胞的长期培养物。所有细胞系都对ABA产生迟发型超敏反应。在抗体形成细胞试验中检测辅助功能时，只有一个细胞系AA3能促进空斑形成细胞的形成。它能够辅助经流感血凝素-球蛋白（FLU-HGG）致敏的B细胞对ABA-钥孔血蓝蛋白-流感病毒（ABA-KLH-FLU）的抗流感反应。这种辅助作用需要ABA和FLU决定簇的连接，因为用ABA-KLH和三硝基苯-KLH-FLU混合物刺激的培养物中未显示空斑形成细胞增加。这些实验证明了在一个消除了被动吸收抗体参与半抗原识别的系统中存在半抗原特异性辅助作用。
