氟脱氧尿苷在体外用于增加125I-碘脱氧尿苷对组织培养细胞摄取时的毒性。

The toxicity of fluorodeoxyuridine when used to increase the uptake of 125I-iododeoxyuridine into tissue culture cells in vitro.

作者信息

Holmes J M

出版信息

J Comp Pathol. 1983 Oct;93(4):531-9. doi: 10.1016/0021-9975(83)90059-2.

Abstract

The use of fluorodeoxyuridine (FUdR) to increase the uptake of 125I-iododeoxyuridine (125IUdR) into tissue culture cells was investigated. FUdR was found to be chemically toxic in itself to 2 canine tissue culture lines at doses used in the literature to increase 125IUdR uptake. FUdR had a cytocidal effect on one cell line, but a cytostatic effect on the other, so that measurement of only cell viability or death in the latter would not have shown a toxic effect. FUdR was shown to mask the radiotoxic effects of 125IUdR and so its use in investigations of the toxicity of 125IUdR may have produced misleading results.

摘要

研究了使用氟脱氧尿苷（FUdR）来增加125I-碘脱氧尿苷（125IUdR）在组织培养细胞中的摄取情况。发现在文献中用于增加125IUdR摄取的剂量下，FUdR本身对2种犬组织培养细胞系具有化学毒性。FUdR对一种细胞系具有杀细胞作用，但对另一种细胞系具有细胞抑制作用，因此仅测量后者的细胞活力或死亡情况不会显示出毒性作用。已证明FUdR会掩盖125IUdR的放射毒性作用，因此其在125IUdR毒性研究中的使用可能产生了误导性结果。