Komatsu-Takaki M
J Biochem. 1983 Oct;94(4):1095-100. doi: 10.1093/oxfordjournals.jbchem.a134452.
ADP binding brought about by inorganic phosphate addition (Pi-dependent ADP binding) on membrane-bound chloroplast coupling factor was studied and the following results were obtained. Under energization by illumination or by acid-base transition, Pi brought about the binding of ADP with an apparent Km value of 0.22 mM. This effect of Pi was lost rapidly after turning the light off or after acid to base transition, concomitant with the loss of ATP synthesizing activity. Pi-dependent ADP binding was inhibited by phlorizin to nearly the same extent as was ATP synthesis. The inhibitory effects of phlorizin on both the Pi-dependent ADP binding and ATP synthesis increased with the decrease of Pi concentration. These results suggest that the Pi-dependent ADP binding reaction participates in the ATP synthesis reaction and that phlorizin inhibits the P1 binding process.
研究了无机磷酸盐添加（Pi 依赖性 ADP 结合）对膜结合叶绿体偶联因子引起的 ADP 结合情况，并获得了以下结果。在光照或酸碱转变激发下，Pi 导致 ADP 结合，其表观 Km 值为 0.22 mM。在关闭光照或从酸到碱转变后，Pi 的这种作用迅速丧失，同时伴随着 ATP 合成活性的丧失。根皮苷对 Pi 依赖性 ADP 结合的抑制程度与对 ATP 合成的抑制程度几乎相同。根皮苷对 Pi 依赖性 ADP 结合和 ATP 合成的抑制作用都随着 Pi 浓度的降低而增加。这些结果表明，Pi 依赖性 ADP 结合反应参与了 ATP 合成反应，并且根皮苷抑制了 Pi 结合过程。
