Kinet J P, Perez-Montfort R, Metzger H
Biochemistry. 1983 Dec 6;22(25):5729-32. doi: 10.1021/bi00294a008.
The receptor on rat basophilic leukemia and related normal cells that binds monomeric immunoglobulin E (IgE) with high affinity contains four polypeptide chains: alpha (to which the IgE binds), beta, and a disulfide-linked dimer of gamma chains. In this study, we have analyzed a further component variably seen when the purified receptors are analyzed on polyacrylamide gels. This component has an apparent Mr of approximately 43 000 and, after treatment with reducing agents, yields one beta and two gamma chains. This complex is generated by immunoprecipitation of preparations totally lacking in it. This novel in vitro phenomenon has provided additional information about the structure of the receptor. Its possible relationship to in vivo aggregation that triggers degranulation of the cells is of interest.
大鼠嗜碱性白血病细胞及相关正常细胞上以高亲和力结合单体免疫球蛋白E（IgE）的受体包含四条多肽链：α链（IgE与之结合）、β链以及由γ链通过二硫键连接而成的二聚体。在本研究中，我们分析了纯化的受体在聚丙烯酰胺凝胶上进行分析时偶尔可见的另一种成分。该成分的表观分子量约为43000，在用还原剂处理后，产生一条β链和两条γ链。这种复合物是通过对完全不含它的制剂进行免疫沉淀产生的。这种新的体外现象为受体结构提供了更多信息。它与触发细胞脱颗粒的体内聚集的可能关系令人关注。
