Divakaran S, Wangel A G
Immunobiology. 1983 Dec;165(5):500-7. doi: 10.1016/S0171-2985(83)80072-2.
T lymphocytes may be separated into theophylline-sensitive (Tsens) and theophylline-resistant (Tres) fractions based on their loss or retention of ERFC-forming ability after incubation with 3 mM theophylline. It has been claimed that Tsens cells have a predominant suppressor function, whilst Tres cells have mainly helper activity. We have studied the sensitive and resistant fractions to ascertain their degree of homogeneity with regard to putative markers for helper and suppressor function. Incubation with theophylline caused no significant change in the expression of OKT3, OKT4, OKT8, and OKM1 antigens. Compared to Tsens, Tres contained significantly more cells expressing OKT4 or Leu 3A antigens and cells bearing Fc mu receptors or containing Gall bodies. The Tsens fraction was enriched for cells with Fc gamma receptors but not for cells expressing OKT8 or LEU 2A. The results suggest that the Tres fraction is enriched for cells with putative helper markers and that the Tsens fraction is enriched for cells with some suppressor markers but also contains a large number of cells of monocytic lineage. However, the two fractions are not homogeneous with respect to Fc receptor status, presence of Gall bodies or antigens defined by OKT or Leu antisera, hence the use of theophylline sensitivity as a means of estimating the sizes of the helper and suppressor populations does not seem advisable.
根据与3 mM茶碱孵育后形成ERFC能力的丧失或保留情况，T淋巴细胞可分为对茶碱敏感（Tsens）和对茶碱耐药（Tres）的部分。据称，Tsens细胞具有主要的抑制功能，而Tres细胞主要具有辅助活性。我们研究了敏感和耐药部分，以确定它们在辅助和抑制功能的假定标志物方面的同质性程度。与茶碱孵育后，OKT3、OKT4、OKT8和OKM1抗原的表达没有显著变化。与Tsens相比，Tres含有显著更多表达OKT4或Leu 3A抗原的细胞以及带有Fcμ受体或含有加尔氏体的细胞。Tsens部分富含具有Fcγ受体的细胞，但不富含表达OKT8或LEU 2A的细胞。结果表明，Tres部分富含具有假定辅助标志物的细胞，而Tsens部分富含具有一些抑制标志物的细胞，但也包含大量单核细胞系细胞。然而，这两个部分在Fc受体状态、加尔氏体的存在或由OKT或Leu抗血清定义的抗原方面并不均匀，因此使用茶碱敏感性作为估计辅助和抑制群体大小的方法似乎不可取。
