分离的大鼠胰岛的ADP核糖基化作用。

ADP-ribosylation of isolated rat islets of Langerhans.

作者信息

Bernofsky C, Amamoo D G

出版信息

Biochem Biophys Res Commun. 1984 Jan 30;118(2):663-8. doi: 10.1016/0006-291x(84)91354-8.

Abstract

Incubation of isolated rat islets of Langerhans with [adenine-2,8-3H]NAD+ results in rapid incorporation of 3H into acid-insoluble products. The major site of incorporation appears to be the cell membrane. The reaction is inhibited by nicotinamide, an ADP-ribosylation inhibitor, and stimulated by arginine, an ADP-ribose acceptor. The results demonstrate that islet membrane proteins can be ADP-ribosylated in the absence of exogenous ADP-ribosylating agents and suggest that ADP-ribosylation plays a role in pancreatic islet cell function.

摘要

将分离的大鼠胰岛与[腺嘌呤-2,8-³H]NAD⁺一起温育，结果³H迅速掺入酸不溶性产物中。掺入的主要部位似乎是细胞膜。该反应受到烟酰胺（一种ADP-核糖基化抑制剂）的抑制，并受到精氨酸（一种ADP-核糖受体）的刺激。结果表明，在没有外源性ADP-核糖基化剂的情况下，胰岛膜蛋白可以被ADP-核糖基化，这表明ADP-核糖基化在胰岛细胞功能中起作用。