霍乱毒素催化二磷酸腺苷核糖掺入大鼠肝细胞膜以及激活腺苷酸环化酶对鸟苷三磷酸的需求。
Requirement for guanosine triphosphate for cholera-toxin-catalysed incorporation of adenosine diphosphate ribose into rat liver plasma membranes and for activation of adenylate cyclase.
作者信息
Doberska C A, MacPherson A J, Martin B R
出版信息
Biochem J. 1980 Mar 15;186(3):749-54. doi: 10.1042/bj1860749.
Abstract
- Cholera toxin was shown to require the presence of GTP to activate rat liver plasma-membrane adenylate cyclase. ATP did not affect the activation process. 2. Cholera toxin catalysed the incorporation of 32P from NAD labelled in the alpha-phosphate group of the ADP moiety into a rat liver plasma-membrane protein with a subunit mol.wt. of 42 500. This is taken to demonstrate ADP-ribosylation. The ADP-ribosylation of this protein also required GTP and was unaffected by ATP. 3. Nicotinamide inhibited both the activation of adenylate cyclase by cholera toxin and the ADP-ribosylation of the protein of 42 500 subunit mol wt. Neither the activation nor the ADP-ribosylation could be reversed by treatment with nicotinamide in the presence of cholera toxin.
摘要
- 已表明霍乱毒素需要GTP的存在才能激活大鼠肝细胞膜腺苷酸环化酶。ATP不影响激活过程。2. 霍乱毒素催化将来自ADP部分α-磷酸基团标记的NAD中的32P掺入到大鼠肝细胞膜中分子量为42500的一种蛋白质中。这被视为证明了ADP核糖基化。该蛋白质的ADP核糖基化也需要GTP，且不受ATP影响。3. 烟酰胺抑制霍乱毒素对腺苷酸环化酶的激活以及分子量为42500亚基的蛋白质的ADP核糖基化。在霍乱毒素存在的情况下，用烟酰胺处理既不能逆转激活过程，也不能逆转ADP核糖基化。
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