Franc J L, Hovsepian S, Fayet G, Bouchilloux S
Biochem Biophys Res Commun. 1984 Feb 14;118(3):910-5. doi: 10.1016/0006-291x(84)91481-5.
Porcine thyroid cells were cultured with or without thyrotropin for 9 days. It is known that the hormone enhances the synthesis of thyroglobulin, with an increase in the content and stability of its m-RNA. We show in the present work that thyrotropin also stimulates a number of glycosyltransferases diversely situated along the process of N-glycosylation. The most increased was oligosaccharyltransferase, responsible for attachment to nascent peptides of preformed core carbohydrate sequences. The relatively low activity level of oligosaccharyltransferase and its preferential responsiveness to thyrotropin supports the possibility of a regulation point at this enzyme.
猪甲状腺细胞在有或无促甲状腺激素的情况下培养9天。已知该激素可增强甲状腺球蛋白的合成，其信使核糖核酸的含量和稳定性会增加。我们在当前研究中表明，促甲状腺激素还能刺激多种糖基转移酶，这些酶在N-糖基化过程中分布各异。增加最多的是寡糖基转移酶，它负责将预先形成的核心碳水化合物序列连接到新生肽上。寡糖基转移酶相对较低的活性水平及其对促甲状腺激素的优先反应性支持了该酶存在调控点的可能性。
