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黏蛋白合成。III. UDP-N-乙酰葡糖胺：半乳糖β1-3（N-乙酰葡糖胺β1-6）N-乙酰半乳糖胺-R（N-乙酰葡糖胺至半乳糖）β3-N-乙酰葡糖胺基转移酶，一种参与黏蛋白型寡糖延长的猪胃黏膜中的酶。

Mucin synthesis. III. UDP-GlcNAc:Gal beta 1-3(GlcNAc beta 1-6)GalNAc-R (GlcNAc to Gal) beta 3-N-acetylglucosaminyltransferase, an enzyme in porcine gastric mucosa involved in the elongation of mucin-type oligosaccharides.

作者信息

Brockhausen I, Williams D, Matta K L, Orr J, Schachter H

出版信息

Can J Biochem Cell Biol. 1983 Dec;61(12):1322-33. doi: 10.1139/o83-169.

DOI:10.1139/o83-169
PMID:6231087
Abstract

Pig gastric mucosa microsomes have been shown to catalyze the following reaction: UDP-GlcNAc + Gal beta 1-3(GlcNAc beta 1-6)-GalNAc-alpha-R----GlcNAc beta 1-3Gal beta 1-3 (GlcNAc beta 1-6)GalNAc-alpha-R + UDP, where R is o-nitrophenyl or benzyl. The enzyme catalyzing this reaction has been named UDP-GlcNAc:Gal beta 1-3(GlcNAc beta 1-6)GalNAc-R (GlcNAc-R (GlcNAc to Gal) beta 3-N-acetylglucosaminyltransferase. The beta 3-GlcNAc-transferase does not act on Gal beta 1-3GalNAc-alpha-o-nitrophenyl. The beta 3-GlcNAc-transferase requires Mn2+ and Triton X-100 for optimal activity. The Vmax for the microsomal enzyme is 8.7 nmol/mg protein per hour and the Km values are 1.6, 0.9, and 1.2 mM for UDP-GlcNAc and the alpha-o-nitrophenyl and alpha-benzyl derivatives of Gal beta 1-3(GlcNAc beta 1-6)GalNAc, respectively. Pig gastric mucosa microsomes catalyze the transfer of GlcNAc to lactose to form GlcNAc beta 1-3Gal beta 1-4Glc, but fail to transfer GlcNAc to lactosyl ceramide, Gal beta 1-4GlcNAc, or Gal beta 1-4GlcNAc-beta-benzyl.

摘要

猪胃黏膜微粒体已被证明能催化以下反应

UDP - GlcNAc + Galβ1 - 3(GlcNAcβ1 - 6)-GalNAc - α - R→GlcNAcβ1 - 3Galβ1 - 3(GlcNAcβ1 - 6)GalNAc - α - R + UDP，其中R为邻硝基苯基或苄基。催化此反应的酶被命名为UDP - GlcNAc:Galβ1 - 3(GlcNAcβ1 - 6)GalNAc - R（GlcNAc - R（GlcNAc到Gal）β3 - N - 乙酰葡糖胺基转移酶）。β3 - GlcNAc转移酶对Galβ1 - 3GalNAc - α - 邻硝基苯基无作用。β3 - GlcNAc转移酶需要Mn2 +和Triton X - 100以达到最佳活性。微粒体酶的Vmax为每小时每毫克蛋白质8.7 nmol，UDP - GlcNAc以及Galβ1 - 3(GlcNAcβ1 - 6)GalNAc的α - 邻硝基苯基和α - 苄基衍生物的Km值分别为1.6、0.9和1.2 mM。猪胃黏膜微粒体催化将GlcNAc转移至乳糖形成GlcNAcβ1 - 3Galβ1 - 4Glc，但无法将GlcNAc转移至乳糖神经酰胺、Galβ1 - 4GlcNAc或Galβ1 - 4GlcNAc - β - 苄基。

相似文献

1
Mucin synthesis. III. UDP-GlcNAc:Gal beta 1-3(GlcNAc beta 1-6)GalNAc-R (GlcNAc to Gal) beta 3-N-acetylglucosaminyltransferase, an enzyme in porcine gastric mucosa involved in the elongation of mucin-type oligosaccharides.黏蛋白合成。III. UDP-N-乙酰葡糖胺：半乳糖β1-3（N-乙酰葡糖胺β1-6）N-乙酰半乳糖胺-R（N-乙酰葡糖胺至半乳糖）β3-N-乙酰葡糖胺基转移酶，一种参与黏蛋白型寡糖延长的猪胃黏膜中的酶。
Can J Biochem Cell Biol. 1983 Dec;61(12):1322-33. doi: 10.1139/o83-169.
2
Mucin synthesis. The action of pig gastric mucosal UDP-GlcNAc:Gal beta 1-3(R1)GalNAc-R2 (GlcNAc to Gal) beta 3-N-acetylglucosaminyltransferase on high molecular weight substrates.粘蛋白合成。猪胃黏膜UDP-N-乙酰葡糖胺：β-半乳糖1-3（R1）N-乙酰半乳糖胺-R2（葡糖胺到半乳糖）β3-N-乙酰葡糖胺基转移酶对高分子量底物的作用。
Can J Biochem Cell Biol. 1984 Nov;62(11):1081-90. doi: 10.1139/o84-140.
3
Mucin synthesis. Conversion of R1-beta 1-3Gal-R2 to R1-beta 1-3(GlcNAc beta 1-6)Gal-R2 and of R1-beta 1-3GalNAc-R2 to R1-beta 1-3(GlcNAc beta 1-6)GalNAc-R2 by a beta 6-N-acetylglucosaminyltransferase in pig gastric mucosa.黏蛋白合成。猪胃黏膜中的β6-N-乙酰氨基葡萄糖转移酶将R1-β1-3Gal-R2转化为R1-β1-3(GlcNAcβ1-6)Gal-R2，以及将R1-β1-3GalNAc-R2转化为R1-β1-3(GlcNAcβ1-6)GalNAc-R2。
Eur J Biochem. 1986 Jun 16;157(3):463-74. doi: 10.1111/j.1432-1033.1986.tb09690.x.
4
Mucin synthesis. II. Substrate specificity and product identification studies on canine submaxillary gland UDP-GlcNAc:Gal beta 1-3GalNAc(GlcNAc leads to GalNAc) beta 6-N-acetylglucosaminyltransferase.粘蛋白合成。II. 犬颌下腺UDP-N-乙酰葡糖胺：β1-3半乳糖基-N-乙酰半乳糖胺（N-乙酰葡糖胺→N-乙酰半乳糖胺）β6-N-乙酰葡糖胺基转移酶的底物特异性及产物鉴定研究
J Biol Chem. 1980 Dec 10;255(23):11253-61.
5
Mucin synthesis. UDP-GlcNAc:GalNAc-R beta 3-N-acetylglucosaminyltransferase and UDP-GlcNAc:GlcNAc beta 1-3GalNAc-R (GlcNAc to GalNAc) beta 6-N-acetylglucosaminyltransferase from pig and rat colon mucosa.黏蛋白合成。来自猪和大鼠结肠黏膜的UDP - N - 乙酰葡糖胺：GalNAc - Rβ3 - N - 乙酰葡糖胺基转移酶和UDP - N - 乙酰葡糖胺：GlcNAcβ1 - 3GalNAc - R（GlcNAc到GalNAc）β6 - N - 乙酰葡糖胺基转移酶
Biochemistry. 1985 Apr 9;24(8):1866-74. doi: 10.1021/bi00329a010.
6
UDP-GlcNAc: Gal beta 3GalNAc-mucin: (GlcNAc----GalNAc) beta 6-N-acetylglucosaminyltransferase and UDP-GlcNAc: Gal beta 3(GlcNAc beta 6) GalNAc-mucin (GlcNAc----Gal)beta 3-N-acetylglucosaminyltransferase from swine trachea epithelium.来自猪气管上皮的UDP-N-乙酰葡糖胺：β3半乳糖基-β-N-乙酰半乳糖胺-粘蛋白：（N-乙酰葡糖胺→N-乙酰半乳糖胺）β6-N-乙酰葡糖胺基转移酶和UDP-N-乙酰葡糖胺：β3（β6-N-乙酰葡糖胺）半乳糖基-β-N-乙酰半乳糖胺-粘蛋白（N-乙酰葡糖胺→半乳糖）β3-N-乙酰葡糖胺基转移酶
Mol Cell Biochem. 1991 Mar 13;101(2):125-43. doi: 10.1007/BF00229530.
7
The separation by liquid chromatography (under elevated pressure) of phenyl, benzyl, and O-nitrophenyl glycosides of oligosaccharides. Analysis of substrates and products for four N-acetyl-D-glucosaminyl-transferases involved in mucin synthesis.通过（高压）液相色谱法分离寡糖的苯基、苄基和邻硝基苯基糖苷。分析参与粘蛋白合成的四种N-乙酰-D-氨基葡萄糖基转移酶的底物和产物。
Carbohydr Res. 1983 Aug 16;120:3-16. doi: 10.1016/0008-6215(83)88002-1.
8
Mucin synthesis. I. Detection in canine submaxillary glands of an N-acetylglucosaminyltransferase which acts on mucin substrates.黏蛋白合成。I. 在犬下颌下腺中检测到一种作用于黏蛋白底物的N-乙酰葡糖胺基转移酶。
J Biol Chem. 1980 Dec 10;255(23):11247-52.
9
Mucin biosynthesis: purification and characterization of a mucin beta 6N-acetylglucosaminyltransferase.黏蛋白生物合成：一种黏蛋白β6-N-乙酰氨基葡萄糖转移酶的纯化与特性分析
J Biol Chem. 1991 Dec 15;266(35):23863-71.
10
Processing O-glycan core 1, Gal beta 1-3GalNAc alpha-R. Specificities of core 2, UDP-GlcNAc: Gal beta 1-3 GalNAc-R(GlcNAc to GalNAc) beta 6-N-acetylglucosaminyltransferase and CMP-sialic acid: Gal beta 1-3GalNAc-R alpha 3-sialyltransferase.O-聚糖核心1（Galβ1-3GalNAcα-R）的加工。核心2的特异性，UDP-N-乙酰葡糖胺：Galβ1-3GalNAc-R（从GlcNAc到GalNAc）β6-N-乙酰葡糖胺基转移酶和CMP-唾液酸：Galβ1-3GalNAc-Rα3-唾液酸转移酶。
Glycoconj J. 1993 Oct;10(5):381-94. doi: 10.1007/BF00731043.

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UDP-GlcNAc: Gal beta 3GalNAc-mucin: (GlcNAc----GalNAc) beta 6-N-acetylglucosaminyltransferase and UDP-GlcNAc: Gal beta 3(GlcNAc beta 6) GalNAc-mucin (GlcNAc----Gal)beta 3-N-acetylglucosaminyltransferase from swine trachea epithelium.来自猪气管上皮的UDP-N-乙酰葡糖胺：β3半乳糖基-β-N-乙酰半乳糖胺-粘蛋白：（N-乙酰葡糖胺→N-乙酰半乳糖胺）β6-N-乙酰葡糖胺基转移酶和UDP-N-乙酰葡糖胺：β3（β6-N-乙酰葡糖胺）半乳糖基-β-N-乙酰半乳糖胺-粘蛋白（N-乙酰葡糖胺→半乳糖）β3-N-乙酰葡糖胺基转移酶
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