外源性胸苷对脆性X表达的体外逆转作用。
In vitro reversal of fragile-X expression by exogenous thymidine.
作者信息
Gardiner G B, Wenger S L, Steele M W
出版信息
Clin Genet. 1984 Feb;25(2):135-9. doi: 10.1111/j.1399-0004.1984.tb00475.x.
Abstract
A virtually thymidine free medium was used to test the effects of exogenous thymidine on lymphocyte expression of the fragile-X. De novo pathway thymidylic acid synthesis in the cells was blocked by FUdR. Our results suggest that in vitro exogenous thymidine is directly responsible for suppressing expression of the fragile-X. More importantly, delayed addition of exogenous thymidine can negate fragile-X expression after it has first been induced by FUdR.
摘要
使用一种几乎不含胸苷的培养基来测试外源性胸苷对脆性X染色体在淋巴细胞中表达的影响。细胞中的从头途径胸苷酸合成被氟尿嘧啶核苷（FUdR）阻断。我们的结果表明，体外的外源性胸苷直接导致脆性X染色体表达受到抑制。更重要的是，在脆性X染色体首先被氟尿嘧啶核苷诱导表达后，延迟添加外源性胸苷可以消除其表达。
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