（4-¹³C）NAD⁺的合成与性质。通过¹³C核磁共振光谱观察其与酵母乙醇脱氢酶的结合。
Synthesis and properties of (4-13C)NAD+. Observation of its binding to yeast alcohol dehydrogenase by 13C-NMR spectroscopy.
作者信息
Oberfrank M, Hull W E, Retey J
出版信息
Eur J Biochem. 1984 Apr 2;140(1):157-61. doi: 10.1111/j.1432-1033.1984.tb08080.x.
Starting from (13C)formic acid, acetone and cyanoacetamide samples of (4-13C)nicotinic acid and (4-13C)-nicotinamide were synthesised in an overall and additive yield of 11%. 1H-NMR and mass spectroscopy showed 90% enrichment of 13C in the expected position. NADase-catalysed exchange between thionicotinamide-adenine dinucleotide and (4-13C)nicotinamide furnished (4-13C)NAD+ which was purified, characterized and quantified by 1H-NMR and 13C-NMR spectroscopy and by enzymic assay. The 13C-NMR signal of (4-13C)beta-NAD+ (146.09 ppm) was broadened and shifted (147.83 ppm) upon binding to yeast alcohol dehydrogenase.
从（13C）甲酸、丙酮和氰基乙酰胺开始，合成了（4-13C）烟酸和（4-13C）烟酰胺样品，总收率和加成收率为11%。1H核磁共振和质谱显示13C在预期位置的富集度为90%。硫代烟酰胺腺嘌呤二核苷酸与（4-13C）烟酰胺之间的NAD酶催化交换产生了（4-13C）NAD+，通过1H核磁共振、13C核磁共振光谱和酶法测定对其进行了纯化、表征和定量。（4-13C）β-NAD+（146.09 ppm）的13C核磁共振信号在与酵母乙醇脱氢酶结合后变宽并发生位移（147.83 ppm）。
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