Crie J S, Morton P, Wildenthal K
J Mol Cell Cardiol. 1983 Aug;15(8):487-94. doi: 10.1016/0022-2828(83)90324-3.
The specific activity of cardiac cathepsin B is significantly decreased by starvation and corticosteroid treatment in vivo, and by exposure of the heart in vitro to insulin, hydrocortisone and cycloheximide. Increases in cathepsin B activity occur following isoproterenol-induced cardiac damage in vivo and exposure in vitro to sucrose. Cathepsin B activity in heart is not changed during normal aging or in thyrotoxicosis. These responses are different from simultaneous changes in cardiac cathepsin D activity in several instances (starvation, corticosteroid treatment, aging and thyrotoxicosis). In the past, measurements of cathepsin D activity in heart have sometimes been considered to be representative of lysosomal proteinase activity in general and used as an index of cardiac lysosomal proteolytic capacity. The present results suggest that changes in cathepsin D do not necessarily reflect alterations in other lysosomal proteinases and may not serve as a valid indicator of overall lysosomal proteolytic capacity under all conditions.
在体内，饥饿和皮质类固醇治疗可显著降低心脏组织蛋白酶B的比活性，在体外，心脏暴露于胰岛素、氢化可的松和环己酰亚胺也会使其比活性降低。在体内，异丙肾上腺素诱导心脏损伤后以及在体外将心脏暴露于蔗糖后，组织蛋白酶B的活性会增加。在正常衰老过程或甲状腺毒症期间，心脏中组织蛋白酶B的活性没有变化。在某些情况下（饥饿、皮质类固醇治疗、衰老和甲状腺毒症），这些反应与心脏组织蛋白酶D活性的同时变化不同。过去，心脏中组织蛋白酶D活性的测量有时被认为是一般溶酶体蛋白酶活性的代表，并被用作心脏溶酶体蛋白水解能力的指标。目前的结果表明，组织蛋白酶D的变化不一定反映其他溶酶体蛋白酶的改变，在所有情况下可能都不能作为整体溶酶体蛋白水解能力的有效指标。
