Weniger J P, Chouraqui J, Zeis A
Reprod Nutr Dev (1980). 1984;24(2):155-64.
The aim of this study was to determine whether the chick embryo testis was able to convert dehydroepiandrosterone and androstenedione into testosterone. Testes from 17-19 day old chick embryos were cultured in vitro in the presence of tritiated dehydroepiandrosterone or androstenedione of high specific activity, and the radioactive testosterone formed was isolated by thin-layer chromatography and identified by recrystallization to constant specific activity. Parallel experiments with mouse embryo testes showed that these methods were valid. However, since specific activity with chick embryo testes was very low and could not be brought to constancy, testosterone could not be identified with these testes. It is concluded that the 17-19 day old chick embryo testis cannot form testosterone from either dehydroepiandrosterone or androstenedione. This conclusion is in keeping with the absence of a physiological role for testosterone in the male chick embryo.
本研究的目的是确定鸡胚睾丸是否能够将脱氢表雄酮和雄烯二酮转化为睾酮。将17 - 19日龄鸡胚的睾丸在含有高比活性的氚标记脱氢表雄酮或雄烯二酮的条件下进行体外培养，通过薄层色谱法分离形成的放射性睾酮，并通过重结晶使其比活性恒定来进行鉴定。对小鼠胚胎睾丸进行的平行实验表明这些方法是有效的。然而，由于鸡胚睾丸的比活性非常低且无法达到恒定，因此无法用这些睾丸鉴定出睾酮。得出的结论是，17 - 19日龄的鸡胚睾丸不能从脱氢表雄酮或雄烯二酮形成睾酮。这一结论与睾酮在雄性鸡胚中缺乏生理作用相一致。
