Claësson M H, Nissen M H, Röpke C
Scand J Immunol. 1984 Mar;19(3):205-10.
Rat spleen cell cultures exposed for 24 h to concanavalin A (Con A-CM) contain, in addition to interleukin 2 (IL-2), factors that promote colony formation in vitro by mouse T cells (TCPA) and B cells (BCPA). TCPA and BCPA are separable on a Sephadex G-75 column. TCPA has a molecular weight of 15,000 daltons and shows the same elution profile as IL-2. Absorption studies with Con A-activated T cells suggested that TCPA and IL-2 are the same entity. BCPA has an apparent molecular weight of 45,000 daltons and stimulates colony formation by B lymphocytes seeded at very low cell density (10(4) - 5 X 10(4) cells/ml). In contrast to TCPA, BCPA can only be demonstrated in gel-filtered material owing to the presence of B colony suppressor activities in crude Con A-CM. Two B colony inhibitory activities were demonstrated by AcA 34 chromatography of crude Con A-CM with molecular weights of 80,000-130,000 and about 50,000, respectively. Because of the specificity, simplicity and sensitivity of B and T colony formation these assay systems should be valuable tools for in vitro testing of biological activities regulating the immune system.
暴露于伴刀豆球蛋白A（Con A-CM）24小时的大鼠脾细胞培养物中，除了白细胞介素2（IL-2）之外，还含有促进小鼠T细胞（TCPA）和B细胞（BCPA）体外集落形成的因子。TCPA和BCPA在葡聚糖G-75柱上可分离。TCPA的分子量为15,000道尔顿，洗脱曲线与IL-2相同。用Con A激活的T细胞进行的吸收研究表明，TCPA和IL-2是同一实体。BCPA的表观分子量为45,000道尔顿，可刺激以非常低的细胞密度（10⁴ - 5×10⁴个细胞/毫升）接种的B淋巴细胞的集落形成。与TCPA不同，由于粗制Con A-CM中存在B集落抑制活性，BCPA只能在凝胶过滤材料中检测到。通过对粗制Con A-CM进行AcA 34层析，证明了两种B集落抑制活性，分子量分别为80,000 - 130,000和约50,000。由于B和T集落形成的特异性、简便性和敏感性，这些检测系统应该是体外测试调节免疫系统生物活性的有价值工具。
