Frémont P H, le Ray C F, le Douarin G H
Cell Differ. 1983 Dec;13(4):325-39. doi: 10.1016/0045-6039(83)90043-x.
Slow anterior latissimus dorsi (ALD) and fast posterior latissimus dorsi (PLD) muscles of 9-day-old quail embryos were cultured in vitro without neurons for 1 to 12 weeks. Several differences could be observed between ALD- and PLD-derived cells. PLD cultures proliferated less rapidly than ALD cultures. ALD-derived muscle fibres exhibited wide Z lines, numerous mitochondria, and a poorly developed sarcotubular system, while PLD-derived muscle fibres exhibited narrow Z lines, few mitochondria, and an abundant sarcotubular system. Staining for myofibrillar ATPase revealed that all well-differentiated ALD-derived muscle fibres were of the beta' type, while PLD-derived fibres were of beta and beta R types. These results show that myoblasts from slow and fast muscle rudiments can express in vitro some of the characteristic features of slow and fast muscle fibres, independently of motor innervation.
将9日龄鹌鹑胚胎的慢型背阔肌（ALD）和快型背阔肌（PLD）在无神经元的情况下进行体外培养1至12周。在源自ALD和PLD的细胞之间可观察到若干差异。PLD培养物的增殖速度比ALD培养物慢。源自ALD的肌纤维呈现宽Z线、大量线粒体以及发育不良的肌管系统，而源自PLD的肌纤维呈现窄Z线、少量线粒体以及丰富的肌管系统。肌原纤维ATP酶染色显示，所有分化良好的源自ALD的肌纤维均为β'型，而源自PLD的纤维为β型和βR型。这些结果表明，来自慢肌和快肌原基的成肌细胞在体外可表达慢肌和快肌纤维的一些特征，与运动神经支配无关。
