Hunt J S, Manning L S, Wood G W
Cell Immunol. 1984 May;85(2):499-510. doi: 10.1016/0008-8749(84)90262-4.
The mechanisms by which the fetal allograft is protected from a maternal anti-fetal immune response are not understood. This study was designed to examine the possibility that tissues near the developing fetus contain immunoregulatory cells and to begin the process of identification of those cells. Dispersed uterine cell suspensions from pregnant Swiss/Webster mice consistently inhibited the responses of normal murine spleen cells to the polyclonal mitogen phytohemagglutinin (PHA). These suspensions contained few lymphocytes (mean 1%), but abundant macrophages (mean 28%), identified by morphology and Fc gamma-receptor expression. Depletion of Fc gamma-receptor-positive cells restored spleen cell (SC) responses to PHA to near normal levels and partial depletion of adherent cells provided varying degrees of relief of the observed suppression. Adherent cells (greater than 95% macrophages) recovered from plastic surfaces were highly immunosuppressive. Suppressor cells appeared to interfere with both early and late stages of spleen cell proliferative responses. The results suggest that cells with some characteristics of macrophages within tissues near the maternal-fetal interface may create a local environment prohibitive to maternal lymphocyte proliferation.
胎儿同种异体移植物免受母体抗胎儿免疫反应影响的机制尚不清楚。本研究旨在探讨发育中胎儿附近的组织中是否含有免疫调节细胞，并开始对这些细胞进行鉴定。来自怀孕的瑞士/韦伯斯特小鼠的分散子宫细胞悬液始终抑制正常小鼠脾细胞对多克隆有丝分裂原植物血凝素（PHA）的反应。这些悬液中淋巴细胞很少（平均1%），但巨噬细胞丰富（平均28%），通过形态学和Fcγ受体表达鉴定。Fcγ受体阳性细胞的去除使脾细胞（SC）对PHA的反应恢复到接近正常水平，贴壁细胞的部分去除提供了不同程度的缓解所观察到的抑制作用。从塑料表面回收的贴壁细胞（大于95%为巨噬细胞）具有高度免疫抑制作用。抑制细胞似乎干扰脾细胞增殖反应的早期和晚期阶段。结果表明，母胎界面附近组织中具有某些巨噬细胞特征的细胞可能会创造一个不利于母体淋巴细胞增殖的局部环境。
