Perinatology Research Branch, Division of Obstetrics and Maternal-Fetal Medicine, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, U.S. Department of Health and Human Services (NICHD/NIH/DHHS), Detroit, MI, 48201, USA.
Department of Obstetrics and Gynecology, Wayne State University School of Medicine, 275 E. Hancock, Detroit, MI, 48201, USA.
Inflamm Res. 2022 Jun;71(5-6):653-668. doi: 10.1007/s00011-022-01569-z. Epub 2022 Apr 21.
To comprehensively characterize monocyte and neutrophil responses to E. coli and its product [lipopolysaccharide (LPS) or endotoxin] in vitro during pregnancy.
Peripheral blood was collected from pregnant women during the third trimester (n = 20) and from non-pregnant women (n = 20).
The number, phagocytic activity, and reactive oxygen species (ROS) production of peripheral monocytes and neutrophils were investigated using flow cytometry. The phenotypes of peripheral monocytes and neutrophils after acute or chronic LPS stimulation were also determined using flow cytometry. Cytokine profiles were quantified for LPS-stimulated peripheral blood mononuclear cells (PBMCs) and a whole blood TruCulture system using a multiplex immunoassay.
Increased number, phagocytic activity, and ROS production capacity of monocytes and neutrophils were found in pregnant compared to non-pregnant women. Additionally, specific subsets of pro-inflammatory monocytes (IL-6CD14 or MIP-1αCD14 cells) and neutrophils (IL-1βCD15 or MIP-1βCD15 cells) were increased in pregnant women in response to acute LPS stimulation. Moreover, distinct subsets of intermediate-activated monocytes expressing CD142, IL-6, and IL-1RA were increased in pregnant women upon chronic LPS stimulation. Last, pregnant women displayed a different cytokine profile than non-pregnant women in LPS-stimulated PBMCs and in whole blood.
Pregnancy tailors the immune responses of circulating monocytes and neutrophils to endotoxin, a Gram-negative bacterial product.
全面描述妊娠期间体外大肠杆菌及其产物（脂多糖[LPS]或内毒素）对单核细胞和中性粒细胞的反应。
采集妊娠晚期（n=20）和非妊娠妇女（n=20）外周血。
采用流式细胞术检测外周血单核细胞和中性粒细胞的数量、吞噬活性和活性氧（ROS）产生。采用流式细胞术检测急性或慢性 LPS 刺激后外周血单核细胞和中性粒细胞的表型。采用多重免疫分析法检测 LPS 刺激的外周血单个核细胞（PBMCs）和全血 TruCulture 系统中的细胞因子谱。
与非妊娠妇女相比，妊娠妇女外周血单核细胞和中性粒细胞数量、吞噬活性和 ROS 产生能力增加。此外，急性 LPS 刺激下，妊娠妇女中促炎单核细胞（IL-6CD14 或 MIP-1αCD14 细胞）和中性粒细胞（IL-1βCD15 或 MIP-1βCD15 细胞）的特定亚群增加。此外，慢性 LPS 刺激下，表达 CD142、IL-6 和 IL-1RA 的中间激活单核细胞的特定亚群在妊娠妇女中增加。最后，与非妊娠妇女相比，LPS 刺激的 PBMCs 和全血中的妊娠妇女表现出不同的细胞因子谱。
妊娠使循环单核细胞和中性粒细胞对革兰氏阴性细菌产物内毒素的免疫反应发生了改变。
