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用脱氧核糖核酸酶I有限消化从海胆细胞核中释放的蛋白质的二维凝胶电泳。

Two-dimensional gel electrophoresis of proteins released from sea urchin nuclei by limited digestion with DNase I.

作者信息

Takahashi K

出版信息

FEBS Lett. 1984 Apr 24;169(2):165-8. doi: 10.1016/0014-5793(84)80311-7.

DOI:10.1016/0014-5793(84)80311-7
PMID:6232150
Abstract

The morula and the mesenchyme blastula nuclei contained approx. 30 nuclear proteins which were preferentially released by limited digestion with DNase I, but no proteins were released from sperm nuclei. While most of the proteins released by DNase I digestion were common to the two embryonic stages, 2 and 6 proteins were specific or enriched in morulae and mesenchyme blastulae, respectively.

摘要

桑椹胚和间充质囊胚细胞核含有约30种核蛋白，这些蛋白可通过用DNase I进行有限消化而优先释放，但精子细胞核未释放出任何蛋白。虽然经DNase I消化释放的大多数蛋白在这两个胚胎阶段是共有的，但分别有2种和6种蛋白在桑椹胚和间充质囊胚中是特异的或富集的。

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FEBS Lett. 1984 Apr 24;169(2):165-8. doi: 10.1016/0014-5793(84)80311-7.
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