Seki Y
Nihon Geka Gakkai Zasshi. 1983 Jun;84(6):488-99.
The specific cellular immunity of renal transplant recipients was investigated by MLR & CML. MLR has been thought to reflect the disparity of HLA-D locus between two individuals and CML to represent an in vitro model for graft rejection and graft adaptation. Anti-donor and anti-control reactivities were diminished in MLR immediately after transplantation even during acute rejection. These MLR suppression are probably due to immuno-suppressive drugs. In acute rejection episode, CML reactivity against donor was elevated and well correlated with clinical findings, but on the other hand, MLR reactivity was suppressed. This discrepancy indicates that strong MLR is not needed to induce high CML. The in vitro development of cytotoxic T cells as a result of proliferation in MLR is impaired in long term survivors and quiescent recipients after transplantation. These suppression is directed to specific donor but not to unrelated third party cells. I have demonstrated the MHC restricted CML suppressor T cell by using HLA identical monozygous twin's lymphocyte. This suppressor T cell may prove to be of value in enhancing graft acceptance and play a part of immunomodulations.
采用混合淋巴细胞反应（MLR）和细胞介导的淋巴细胞毒试验（CML）对肾移植受者的特异性细胞免疫进行了研究。人们一直认为MLR反映了两个个体之间HLA - D位点的差异，而CML代表了移植排斥和移植适应的体外模型。即使在急性排斥反应期间，移植后即刻的MLR中抗供体和抗对照反应性也会降低。这些MLR抑制可能是由于免疫抑制药物所致。在急性排斥反应发作时，针对供体的CML反应性升高，且与临床发现密切相关，但另一方面，MLR反应性受到抑制。这种差异表明，诱导高CML并不需要强烈的MLR。在长期存活者和移植后静止的受者中，由于MLR中细胞增殖导致的细胞毒性T细胞的体外发育受到损害。这些抑制作用针对特定的供体细胞，而不是无关的第三方细胞。我通过使用HLA相同的同卵双胞胎淋巴细胞证明了MHC限制的CML抑制性T细胞。这种抑制性T细胞可能在增强移植接受方面具有价值，并发挥免疫调节作用。
