Macknight A D, Leaf A
Am J Physiol. 1978 Jan;234(1):F1-9. doi: 10.1152/ajprenal.1978.234.1.F1.
The sodium transport pool in epithelial cells represents sodium involved in active transport across the epithelium. There has been much controversy about the size of such a pool and even about its existence. Techniques for estimating the size of this pool are described. By analysis of toad bladder epithelial cells scraped from hemibladders mounted in chambers under a variety of conditions it has proved possible to detect and to quantify a sodium transport pool. Only about 20 percent of non-inulin space sodium measured flame photometrically is contained in this pool. This represents the total sodium entering cells from the mucosal medium, is in good agreement with the cellular sodium measured by the electron microprobe, and averages some 10-16 mmol/kg tissue H2O. Measurements of the pool in other tissues are considered.
上皮细胞中的钠转运池代表参与跨上皮主动转运的钠。关于这个池的大小甚至其存在与否一直存在很多争议。文中描述了估计这个池大小的技术。通过分析在各种条件下安装在小室中的半膀胱刮下的蟾蜍膀胱上皮细胞，已证明能够检测和量化钠转运池。通过火焰光度法测量的非菊粉空间钠中只有约20%包含在这个池中。这代表了从黏膜介质进入细胞的总钠量，与电子微探针测量的细胞内钠量高度一致，平均约为10 - 16 mmol/kg组织H₂O。还考虑了在其他组织中对该池的测量。
