Ferguson E R, Blachley J D, Carter N W, Knochel J P
Am J Physiol. 1984 May;246(5 Pt 2):F700-9. doi: 10.1152/ajprenal.1984.246.5.F700.
Muscle ion composition, Na-K-ATPase activity, tissue respiration, and transmembrane potential differences were measured after 28 and 56 days of ethanol consumption (6.2 g X kg-1 X day-1) or an isocaloric amount of glucose in 12 and 4 dogs, respectively. Ethanol and glucose were given as supplements to an otherwise nutritious diet. After 28 and 56 days of alcohol consumption, skeletal muscle contents of phosphorus, magnesium, and potassium were significantly reduced as compared with either the control values or those in glucose-fed animals. In alcohol-fed animals, muscle sodium chloride, and calcium were significantly elevated. Ethanol consumption also resulted in hyperpolarization of the resting transmembrane potential of skeletal muscle fibers and a significant increase in Na-K-ATPase activity. No change was noted in Mg-ATPase activity. The increase in Na-K-ATPase activity was accompanied by increased sodium transport-dependent respiration. These results indicate that a subclinical myopathy may be induced by alcohol in the dog. Malnutrition did not appear to be a factor in this study, and thus the changes observed are believed to be due to ethanol per se. The magnitude and direction of these changes are similar to those observed in the skeletal muscle of chronically alcoholic humans. The changes in Na-K-ATPase activity and sodium transport-dependent respiration may represent adaptive responses of the muscle cell to ion transport or membrane disorders induced by ethanol.
分别对12只和4只犬给予乙醇（6.2 g×kg⁻¹×天⁻¹）或等热量的葡萄糖，持续28天和56天后，测量其肌肉离子组成、钠钾ATP酶活性、组织呼吸及跨膜电位差。乙醇和葡萄糖均作为营养均衡饮食的补充剂给予。与对照组或喂食葡萄糖的动物相比，饮酒28天和56天后，骨骼肌中的磷、镁和钾含量显著降低。在饮酒的动物中，肌肉中的氯化钠和钙含量显著升高。饮酒还导致骨骼肌纤维静息跨膜电位超极化，钠钾ATP酶活性显著增加。镁ATP酶活性未观察到变化。钠钾ATP酶活性的增加伴随着钠转运依赖性呼吸的增加。这些结果表明，犬可能会因酒精诱发亚临床肌病。在本研究中，营养不良似乎不是一个因素，因此观察到的变化被认为是由乙醇本身引起的。这些变化的程度和方向与在慢性酒精中毒人类骨骼肌中观察到的相似。钠钾ATP酶活性和钠转运依赖性呼吸的变化可能代表肌肉细胞对乙醇诱导的离子转运或膜紊乱的适应性反应。
