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[人类精子细胞核的体外解凝聚及图像分析]

[In vitro decondensation of the human spermatozoan nucleus and image analysis].

作者信息

Pasteur X, Laurent J L, Azéma J, Jourlin M, Deage P, Grange D

出版信息

C R Seances Soc Biol Fil. 1983;177(4):536-52.

PMID:6232987
Abstract

Between the first stage: differentiation of the human spermatozoa from spermatid in testicle, and the second one: formation of male pronucleus in ovum, the nucleus of spermatozoa undergoes a chromatin condensation and a chromatin decondensation mechanism. Besides it is possible to get this decondensation mechanism in vitro by putting spermatozoa into specific solution with the purpose of carry out an image analysis. The aim of this study was to manage a quantitative analysis of the in vitro nuclear chromatin decondensation of human spermatozoa with an image analyzer of T.A.S. "texture analysis system".

摘要

在第一阶段

人类精子在睾丸中从精子细胞分化出来,与第二阶段:卵子中雄原核形成之间,精子的细胞核经历染色质浓缩和染色质解浓缩机制。此外,通过将精子置于特定溶液中以进行图像分析,有可能在体外获得这种解浓缩机制。本研究的目的是使用T.A.S. “纹理分析系统”图像分析仪对人类精子体外核染色质解浓缩进行定量分析。

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