着色性干皮病：DNA修复内切酶的体外互补作用

Xeroderma pigmentosum: in vitro complementation of DNA repair endonuclease.

作者信息

Helland D, Kleppe R, Lillehaug J R, Kleppe K

出版信息

Carcinogenesis. 1984 Jun;5(6):833-6. doi: 10.1093/carcin/5.6.833.

DOI:10.1093/carcin/5.6.833

Abstract

The DNA repair endonuclease activity has been studied in several xeroderma pigmentosum (XP) cell lines of complementation groups A and C. phi X174 RFI DNA treated with u.v.-light or OsO4 was used as a substrate. All XP cells tested appeared to lack this enzyme compared to control cells. However, when extracts from complementation groups A and C were mixed, activity levels close to that of the control cells were found, clearly indicating that the two cell lines can complement each other with regard to the DNA repair endonuclease activity. The XP cells were found to contain normal levels of AP-endonuclease.

摘要

已在互补组A和C的几种色素性干皮病（XP）细胞系中研究了DNA修复内切核酸酶活性。用紫外线或四氧化锇处理的φX174 RFI DNA用作底物。与对照细胞相比，所有测试的XP细胞似乎都缺乏这种酶。然而，当将互补组A和C的提取物混合时，发现活性水平接近对照细胞，这清楚地表明这两种细胞系在DNA修复内切核酸酶活性方面可以相互互补。发现XP细胞含有正常水平的AP内切核酸酶。

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