Bezvershenko I A, Sinelnikova A L, Boyko M G
Immunol Lett. 1984;7(5):239-42. doi: 10.1016/0165-2478(84)90028-2.
Mouse thymocytes lose the affinity for syngeneic erythrocytes in the rosette formation test after heating in the isotonic medium for 1 h at 45 degrees. The thymocyte receptors for syngeneic erythrocytes appear in the medium after heating. The rosetting of heated and washed thymocytes is restored after incubation with the supernatant obtained from heating in medium thymocytes. The receptors for allogeneic erythrocytes were not separated from thymocytes under the same conditions of heating and washing. The receptors for syngeneic erythrocytes separated by heating can be adsorbed on the column with IgG-Sepharose conjugate and probably are connected with the receptors for Fc portion of IgG.
在45摄氏度的等渗介质中加热1小时后,小鼠胸腺细胞在玫瑰花结形成试验中失去了对同基因红细胞的亲和力。加热后,同基因红细胞的胸腺细胞受体出现在介质中。用加热培养的胸腺细胞获得的上清液孵育后,加热并洗涤过的胸腺细胞的玫瑰花结形成得以恢复。在相同的加热和洗涤条件下,异基因红细胞的受体并未与胸腺细胞分离。通过加热分离的同基因红细胞受体可被IgG-琼脂糖偶联物吸附在柱上,并且可能与IgG的Fc部分受体相连。