Suzuki S, Koide A
Anal Biochem. 1984 Feb;137(1):101-5. doi: 10.1016/0003-2697(84)90353-1.
A simple procedure for the isolation of heparan sulfates from pig lung using a poly-L-lysine-Sepharose column is described. Glycosaminoglycans are absorbed on poly-L-lysine-Sepharose at pH 7.5 and eluted with an NaCl linear gradient in the following order: hyaluronic acid (0.32 M NaCl), chondroitin (0.36 M NaCl), keratan sulfate (0.80 M NaCl), chondroitin 4-sulfate (0.86 M NaCl), chondroitin 6-sulfate (0.95 M NaCl), dermatan sulfate (0.91 M NaCl), heparan sulfate (1.2 M NaCl), and heparin (1.35 M NaCl). Based on these observations, isolation of heparan sulfate from pig lung crude heparan sulfate fractions which contain chondroitin sulfates and dermatan sulfate was attempted, using this chromatographic technique.
本文描述了一种使用聚-L-赖氨酸-琼脂糖柱从猪肺中分离硫酸乙酰肝素的简单方法。糖胺聚糖在pH 7.5时吸附于聚-L-赖氨酸-琼脂糖柱上，并用NaCl线性梯度按以下顺序洗脱：透明质酸（0.32 M NaCl）、软骨素（0.36 M NaCl）、硫酸角质素（0.80 M NaCl）、硫酸软骨素4-硫酸酯（0.86 M NaCl）、硫酸软骨素6-硫酸酯（0.95 M NaCl）、硫酸皮肤素（0.91 M NaCl）、硫酸乙酰肝素（1.2 M NaCl）和肝素（1.35 M NaCl）。基于这些观察结果，尝试使用这种色谱技术从含有硫酸软骨素和硫酸皮肤素的猪肺粗硫酸乙酰肝素馏分中分离硫酸乙酰肝素。
