Hadikusumo R G, Hertzog P J, Marzuki S
Biochim Biophys Acta. 1984 Jun 26;765(3):258-67. doi: 10.1016/0005-2728(84)90164-6.
Fourteen stable lines of myeloma-spleen cell hybrids producing antibodies against the mitochondrial H+-ATPase have been isolated. One reacted with the alpha-subunit of the enzyme complex (Mr 56000), nine with the beta-subunit (Mr 54000), and four with a 25 kDa subunit which has not been previously characterized. These antibodies are inhibitory or stimulatory or have no effect upon the enzyme activity. Two of the monoclonal anti-beta-subunit antibodies were found to be particularly effective in immunoprecipitating intact H+-ATPase complex.
已分离出14株产生抗线粒体H⁺-ATP酶抗体的骨髓瘤-脾细胞杂交稳定株系。其中一株与酶复合物的α亚基（分子量56000）发生反应，9株与β亚基（分子量54000）发生反应，4株与一个以前未鉴定过的25kDa亚基发生反应。这些抗体对酶活性具有抑制、刺激或无作用。发现两种抗β亚基单克隆抗体在免疫沉淀完整的H⁺-ATP酶复合物方面特别有效。
