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[利用纸片法开发一种定量评估微生物对抗生素敏感性的方法。研究用于确定微生物对抗生素敏感性的不同营养培养基]

[Development of a method for the quantitative assessment of microorganism sensitivity to antibiotics using discs. The study of different nutrient media for determining microorganism sensitivity to antibiotics].

作者信息

Rezvan S P, Chaĭkovskaia S M, Semenov S M, Galkina T P, Dmitrova L I

出版信息

Antibiotiki. 1978 Jan;23(1):50-3.

PMID:623447
Abstract

Five nutrient media used for determination of microbial sensitivity to antibiotics, i.e. beaf-peptone agar, Hottinger pancreatic beaf infusion agar, sprat hydrolysate nutrient agar of the Dagestan Research Institute of Nutrient Media, Muller-Chintone agar from Bulgaria and "Oxoid" agar for determination of microbial sensitivity were studied comparatively. The media were compared with respect to the growth density with the use of different test-cultures and the clearance of the inhibition growth zones around the discs containing different antibiotics. The best results were obtained with the use of sprat hydrolysate nutrient agar. Further studies on the medium standardization are necessary.

摘要

对用于测定微生物对抗生素敏感性的5种营养培养基进行了比较研究,即牛肉蛋白胨琼脂、霍廷格胰蛋白胨牛肉浸液琼脂、达吉斯坦营养培养基研究所的鲱鱼水解物营养琼脂、保加利亚的穆勒-秦托琼脂以及用于测定微生物敏感性的“Oxoid”琼脂。通过使用不同的试验培养物比较了这些培养基的生长密度,并比较了含有不同抗生素的圆片周围抑制生长区的清晰程度。使用鲱鱼水解物营养琼脂获得了最佳结果。有必要对该培养基进行进一步的标准化研究。

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