[用大鼠肝脏染色质中的核酸酶恢复γ射线辐照DNA的引物活性]
[Restoration of the primer activity of gamma-irradiated DNA by nucleases from rat liver chromatin].
作者信息
Beliakova N V, Kleĭner H E, Krutiakov V M
出版信息
Radiobiologiia. 1984 May-Jun;24(3):341-4.
PMID:6234614
Abstract
gamma-Irradiation of DNA results in a several-fold decrease of its primer activity measured as one substrate synthesis catalyzed by DNA polymerase beta. However, the combined treatment of injured DNA with 3'----5' exonuclease and endonuclease I from rat liver chromatin almost normalizes primer activity of DNA. Therefore the above-mentioned nucleases are capable of excising the gamma-injured nucleotides from 3'-OH ends of DNA.
摘要
DNA的γ射线照射会导致其引物活性降低数倍，该活性通过DNA聚合酶β催化的一种底物合成来衡量。然而，用大鼠肝脏染色质中的3'→5'核酸外切酶和核酸内切酶I联合处理受损DNA，几乎可使DNA的引物活性恢复正常。因此，上述核酸酶能够从DNA的3'-OH末端切除γ射线损伤的核苷酸。
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