Lanotte M
Biol Cell. 1984;50(2):107-20. doi: 10.1111/j.1768-322x.1984.tb00257.x.
Collagen, a major component of the extracellular matrix in vivo, has been used as a tridimensional gel matrix for cultured hemopoietic clones. Its resemblance to the natural matrix produced by cells makes it ideal for studies on proliferation and differentiation of hemopoietic lineages. Every lineage, including granulocytes (basophilic, eosinophilic and neutrophilic polymorphs) monocyte-macrophages, megakaryocytes, erythroid and lymphoid lineages could be grown using a standardized collagen medium, provided that specific stimulators were added in the culture. Clones were scored on either live or fixed cultures. Compared to other gel substrates, collagen matrix proved superior for cell proliferation and maturation. Additional advantages (in situ clonal analysis by histological staining, enzyme cytochemistry), and other possibilities of the method are reported and discussed. The system offers great potential for cellular immunology, hematology and molecular biology with peculiar reference to differentiation of normal hemopoietic cells, viral transformation and leukemogenesis in vitro. These applications are reviewed.
胶原蛋白是体内细胞外基质的主要成分，已被用作培养造血克隆的三维凝胶基质。它与细胞产生的天然基质相似，使其成为研究造血谱系增殖和分化的理想选择。每个谱系，包括粒细胞（嗜碱性、嗜酸性和中性多形核细胞）、单核细胞-巨噬细胞、巨核细胞、红系和淋巴系，只要在培养中添加特定的刺激物，都可以使用标准化的胶原蛋白培养基生长。克隆在活细胞培养或固定细胞培养上进行计数。与其他凝胶底物相比，胶原蛋白基质在细胞增殖和成熟方面表现更优。还报告并讨论了该方法的其他优点（通过组织学染色、酶细胞化学进行原位克隆分析）以及其他可能性。该系统在细胞免疫学、血液学和分子生物学方面具有巨大潜力，尤其适用于正常造血细胞的分化、体外病毒转化和白血病发生的研究。本文对这些应用进行了综述。
