Jannin B, Maume B F
C R Seances Soc Biol Fil. 1984;178(1):92-7.
The biosynthesis of prostaglandins of the E pathway was shown in new-born rat adrenocortical cells in long-term primary culture, by direct incubation of labelled arachidonic acid or by incubation with prelabelled cells. A rapid method of extraction was developed using silanised silica cartridges.
通过直接孵育标记的花生四烯酸或与预标记细胞一起孵育,在长期原代培养的新生大鼠肾上腺皮质细胞中显示了E途径前列腺素的生物合成。使用硅烷化硅胶柱开发了一种快速提取方法。