Porzsolt F, Heimpel H
Immunol Lett. 1984;8(1):33-8. doi: 10.1016/0165-2478(84)90101-9.
In addition to the cytotoxic T lymphocytes (CTL) killer cells which lyse autologous PHA lymphoblasts are also generated in mixed lymphocyte cultures (MLC). By modifying various tissue culture conditions, we demonstrate that the generation of allospecific effectors can be reduced but not the lytic activity against autologous PHA lymphoblasts. Cold target inhibition experiments showed a cross reaction between autologous and allogeneic PHA lymphoblasts. No inhibition was seen with fresh lymphocytes. It seems, therefore, that the measured lytic activity of specific CTL generated in a MLC is in fact partially mediated by cytotoxic cells which kill also autologous PHA lymphoblasts.
除了细胞毒性T淋巴细胞（CTL）外，在混合淋巴细胞培养（MLC）中还会产生溶解自体PHA淋巴母细胞的杀伤细胞。通过改变各种组织培养条件，我们证明同种异体特异性效应细胞的产生可以减少，但对自体PHA淋巴母细胞的裂解活性不能减少。冷靶抑制实验表明自体和异体PHA淋巴母细胞之间存在交叉反应。新鲜淋巴细胞未见抑制作用。因此，似乎在MLC中产生的特异性CTL的实测裂解活性实际上部分是由也能杀死自体PHA淋巴母细胞的细胞毒性细胞介导的。
