Sagi-Eisenberg R, Foreman J C
Immunol Lett. 1984;8(1):43-7. doi: 10.1016/0165-2478(84)90103-2.
This communication describes a simple procedure for fractionating mast cells producing plasma membranes and intact granules. Mast cells were purified over a bovine serum albumin density gradient and disrupted under conditions in which no histamine was released. Iodinated immunoglobulin E (IgE) bound to the cells served as a marker for the plasma membrane fraction. Employing a discontinuous sucrose gradient the plasma membrane and granule fractions were separated. The specific activity of the IgE binding to the isolated plasma membrane fractions was 10-fold higher compared with that of the IgE binding to intact cells.
本通讯描述了一种用于分离产生质膜和完整颗粒的肥大细胞的简单程序。肥大细胞通过牛血清白蛋白密度梯度进行纯化，并在不释放组胺的条件下进行破碎。与细胞结合的碘化免疫球蛋白E（IgE）用作质膜部分的标志物。利用不连续蔗糖梯度分离质膜和颗粒部分。与IgE结合完整细胞相比，IgE与分离的质膜部分结合的比活性高10倍。
