Li W, Miller R A
Cell Immunol. 1984 Sep;87(2):366-78. doi: 10.1016/0008-8749(84)90006-6.
In vitro culture of mouse spleen cells for 2 days or more leads to the production of adherent, phagocytic, Thy-1-, Ia+, Lyt-2- cells ("suppressor macrophages") which strongly inhibit the proliferative response of T and B lymphocytes to a variety of stimuli: mitogens, specific antigens, and antigen-nonspecific growth factors. Suppressive activity fails to develop, however, in cultured spleen cells from which nonadherent cells have been removed before the initial 48-hr incubation, and only partial suppression is obtained from cell suspensions from which T cells have been depleted before culture. We find that the requirement for nonadherent cells can be replaced by graded doses of lymphocytes. Lyt-2- and Lyt-2+ T cells are about equally potent in inducing suppressive activity in nonadherent cells. Surprisingly, B cells (containing fewer than 0.1% contaminating T cells) are also able to induce suppression in this system. The suppression induced includes both indomethacin-sensitive and indomethacin-resistant components. Interestingly, not all stages of mitogen-induced T-cell activation are blocked by these adherent cells: proliferation is inhibited, but production of interleukin 2 (IL-2) and interleukin 3 (IL-3) is unaffected.
将小鼠脾细胞进行体外培养2天或更长时间会导致产生贴壁、具有吞噬作用、Thy-1-、Ia+、Lyt-2-细胞（“抑制性巨噬细胞”），这些细胞会强烈抑制T和B淋巴细胞对多种刺激的增殖反应：丝裂原、特异性抗原和抗原非特异性生长因子。然而，在最初48小时培养前已去除非贴壁细胞的培养脾细胞中，抑制活性无法产生，并且从培养前已去除T细胞的细胞悬液中只能获得部分抑制作用。我们发现，对非贴壁细胞的需求可以由不同剂量的淋巴细胞替代。Lyt-2-和Lyt-2+ T细胞在诱导非贴壁细胞的抑制活性方面效力大致相同。令人惊讶的是，B细胞（含污染T细胞少于0.1%）在该系统中也能够诱导抑制作用。所诱导的抑制作用包括对吲哚美辛敏感和对吲哚美辛耐药的成分。有趣的是，这些贴壁细胞并未阻断丝裂原诱导的T细胞活化的所有阶段：增殖受到抑制，但白细胞介素2（IL-2）和白细胞介素3（IL-3）的产生未受影响。
