Grimes P A, McGlinn A, Laties A M, Naji A
Exp Eye Res. 1984 Jun;38(6):569-77. doi: 10.1016/0014-4835(84)90175-1.
Stereological analysis of electron micrographs of the pigment epithelium of rats with drug-induced diabetes demonstrated an increase of plasma membrane surface area at the basal aspect of the cells. In none of the diabetic animals examined was there any evidence of breakdown of the blood-retinal barrier to the protein tracer, horseradish peroxidase. Statistically significant increases in basal plasma membrane length and surface density (surface area per unit cell volume) were measured in both streptozotocin and alloxan-injected rats after four weeks of diabetes. When hyperglycemia in streptozotocin-injected rats was promptly reversed by transplantation of normal pancreatic islets, the increase of membrane surface area did not occur. We conclude, therefore, that increased basal surface area of pigment epithelial cells is related to the diabetic condition rather than to a toxic action of the diabetogenic agents. Furthermore, increased membrane surface area was present in streptozotocin-diabetic rats killed after six months of diabetes indicating that the structural change is relatively stable. Relation of basal membrane alteration in the pigment epithelium to any functional disturbance of the barrier cell layer or of the retina in diabetes remains to be established.
对药物诱导糖尿病大鼠色素上皮细胞电子显微照片进行的体视学分析表明，细胞基底侧质膜表面积增加。在所检查的糖尿病动物中，均未发现有血视网膜屏障对蛋白示踪剂辣根过氧化物酶的破坏迹象。糖尿病四周后，注射链脲佐菌素和四氧嘧啶的大鼠，其基底质膜长度和表面密度（每单位细胞体积的表面积）在统计学上均有显著增加。当通过移植正常胰岛迅速逆转注射链脲佐菌素大鼠的高血糖时，膜表面积并未增加。因此，我们得出结论，色素上皮细胞基底表面积增加与糖尿病状态有关，而非与致糖尿病药物的毒性作用有关。此外，糖尿病六个月后处死的链脲佐菌素诱导糖尿病大鼠存在膜表面积增加的情况，这表明结构变化相对稳定。色素上皮基底膜改变与糖尿病中屏障细胞层或视网膜的任何功能紊乱之间的关系仍有待确定。
