去除组蛋白H1会使染色质中的连接DNA暴露于核酸酶I。
Removal of histone H1 exposes linker DNA in chromatin to DNAse I.
作者信息
Iovcheva C, Mladenova I, Dessev G
出版信息
Mol Biol Rep. 1984 Jul;10(1):9-12. doi: 10.1007/BF00775147.
PMID:6236359
Abstract
After removal of histone H1 about 40% of DNA in chromatin acquires the sensitivity of naked DNA to DNAse I. Digestion of H1-depleted chromatin with DNAse I leads to a qualitative change in the digestion pattern, generating DNA fragments of approx. 200 b.p. and multiples, similar to those obtained with micrococcal nuclease. Both effects are reversed upon reconstitution of purified H1 to H1-depleted chromatin.
摘要
去除组蛋白H1后，染色质中约40%的DNA获得了裸露DNA对核酸酶I的敏感性。用核酸酶I消化去除H1的染色质会导致消化模式发生质的变化，产生约200个碱基对及其倍数的DNA片段，类似于用微球菌核酸酶获得的片段。将纯化的H1重新组装到去除H1的染色质上后，这两种效应均可逆转。
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