Christophe J, Dehaye J P, Winand J
Bibl Nutr Dieta. 1978(26):8-16. doi: 10.1159/000401126.
The methods utilized in our laboratory for a biochemical approach of obesity include dietary manipulations, fatty acid analysis of tissue lipids, in vivo lipogenesis from [3H H2O and [1-14C] acetate, in vitro utilization of [3H] H2O, [U-14C] glucose, [U-14C] fructose, [U-14C] alanine and [1-14C] acetate by adipose tissue fragments, hormone sensitivity (to insulin and catecholamines), and the activity of enzymes such as fatty acid synthetase and adenylate cyclase in adipose tissue extracts. With these methods at hand, it is possible to estimate the major biochemical factors responsible for fat accumulation in adipose tissue. As an example, the case of obese (ob/ob) homozygotic animals of the C57BL/6J strain of Bar Harbor, which suffer from an autosomal recessive obese-hyperglycemic (O-H) syndrome, is compared to that of control nonobese (ob+/ob+) mice from the same strain. The hereditary O-H syndrome in ob/ob mice is characterized by obesity, resistance to the action of insulin, and hyperinsulinism. The development of obesity depends on high lipogenesis in fat depots. Contribute also to obesity a large influx of fatty acids of hepatic and dietary origin, and reduced lipolysis. In these mice, a high fat diet is more propitious to fat accretion than a high-carbohydrate diet.
我们实验室用于肥胖症生化研究方法包括饮食控制、组织脂质的脂肪酸分析、利用[³H]H₂O和[1-¹⁴C]乙酸盐进行体内脂肪生成、脂肪组织片段对[³H]H₂O、[U-¹⁴C]葡萄糖、[U-¹⁴C]果糖、[U-¹⁴C]丙氨酸和[1-¹⁴C]乙酸盐的体外利用、激素敏感性（对胰岛素和儿茶酚胺）以及脂肪组织提取物中脂肪酸合成酶和腺苷酸环化酶等酶的活性。有了这些方法，就有可能估算出导致脂肪组织中脂肪堆积的主要生化因素。例如，将患有常染色体隐性肥胖-高血糖（O-H）综合征的缅因州巴尔港C57BL/6J品系肥胖（ob/ob）纯合子动物与同一品系的对照非肥胖（ob⁺/ob⁺）小鼠进行比较。ob/ob小鼠的遗传性O-H综合征的特征是肥胖、对胰岛素作用的抵抗和高胰岛素血症。肥胖的发展取决于脂肪库中较高的脂肪生成。肝脏和饮食来源的大量脂肪酸流入以及脂肪分解减少也导致了肥胖。在这些小鼠中，高脂肪饮食比高碳水化合物饮食更有利于脂肪堆积。
