Puvion-Dutilleul F, Pédron J, Cajean-Feroldi C
Eur J Cell Biol. 1984 Jul;34(2):313-22.
Immunocytochemical techniques have been applied to cells infected with human adenovirus type 5 in order to study the distribution of antigens related to the virus-encoded Mr = 72000 (72K) DNA-binding protein. In embedded cells 72K antigen was found to be associated with 'clear' fibrillar inclusions, in which viral DNA replication is known to take place. "Dense" fibrillar inclusions, which are thought to contain previously replicated viral DNA, do not contain detectable levels of 72K antigen, suggesting that the conversion of clear inclusions to dense inclusions is accompanied by loss of the 72K protein. In cells examined by the spreading technique of Miller, 72K antigen is associated with the 10 nm "thick filaments" previously described by several laboratories, consistent with the established role of the 72K protein in viral replication complexes.
免疫细胞化学技术已应用于感染人5型腺病毒的细胞，以研究与病毒编码的分子量为72000（72K）的DNA结合蛋白相关的抗原分布。在包埋的细胞中，发现72K抗原与“透明”纤维状内含物相关，已知病毒DNA复制发生在此类内含物中。“致密”纤维状内含物被认为含有先前复制的病毒DNA，其中不含可检测水平的72K抗原，这表明透明内含物向致密内含物的转变伴随着72K蛋白的丢失。在用米勒铺展技术检查的细胞中，72K抗原与几个实验室先前描述的10纳米“粗丝”相关，这与72K蛋白在病毒复制复合体中的既定作用一致。
