在存在极不稳定的磷酸酯的情况下测量磷酸盐浓度。
Measurement of phosphate concentration in the presence of very labile phosphate esters.
作者信息
Lane R J, Watmough N J
出版信息
Anal Biochem. 1984 Aug 1;140(2):424-7. doi: 10.1016/0003-2697(84)90188-x.
There are a number of methods available for the measurement of phosphate ion concentration, which may be used when moderately labile phosphate esters such as ATP are present in low concentration. However, the highly acidic conditions usually employed make these unsuitable when very labile esters such as phosphocreatine are present. A method in which the phosphomolybdate complex is developed under mildly acidic conditions, using high molybdate concentrations to counteract the reduced assay sensitivity at high pH, is described. The assay is linear in the range 5-300 microM phosphate, and micromolar concentrations of phosphate can be reliably measured in the presence of millimolar phosphocreatine.
有多种方法可用于测量磷酸根离子浓度，当存在低浓度的中度不稳定的磷酸酯（如ATP）时可以使用这些方法。然而，当存在极不稳定的酯（如磷酸肌酸）时，通常采用的高酸性条件使得这些方法不适用。本文描述了一种方法，即在弱酸性条件下形成磷钼酸络合物，使用高浓度的钼酸盐来抵消在高pH值下测定灵敏度的降低。该测定在5 - 300微摩尔磷酸根范围内呈线性，并且在存在毫摩尔浓度的磷酸肌酸的情况下，可以可靠地测量微摩尔浓度的磷酸根。
Zotero 插件