5-氟尿苷对双翅目唾液腺细胞RNA标记的差异抑制作用。

Differential inhibitory effect of 5-fluorouridine on RNA labelling in dipteran salivary gland cells.

作者信息

Lönn U

出版信息

Biochim Biophys Acta. 1978 Jan 26;517(1):265-8. doi: 10.1016/0005-2787(78)90054-0.

Abstract

In Chironomus salivary glands, 5-fluorouridine inhibits labelling of ribosomal RNA. The drug does not prevent labelling of other types of RNA- like heterodisperse messenger-like RNA, 75-S RNA of Balbiani ring origin and 4-S RNA, which furthermore are exported to cytoplasm. The potential use of this drug for the study of RNA metabolism in insect salivary glands can be compared with the use of low doses of actinomycin D in cultured mammalian cells.

摘要

在摇蚊唾液腺中，5-氟尿苷抑制核糖体RNA的标记。该药物并不阻止其他类型RNA的标记，如异质分散的类信使RNA、源自巴尔比亚尼环的75-S RNA和4-S RNA，而且这些RNA会输出到细胞质中。这种药物在昆虫唾液腺RNA代谢研究中的潜在用途可与在培养的哺乳动物细胞中使用低剂量放线菌素D的情况相比较。