Taube D, Welsh K, Hobby P, Williams D G
Clin Nephrol. 1984 Sep;22(3):127-32.
In order to study the changes in T lymphocyte subpopulations in both allografts and peripheral blood of patients following renal transplantation, we have examined 72 fine needle allograft aspirates and 56 peripheral blood samples from 24 patients during the first month following transplantation. The patients were studied before, during and after rejection episodes. Monoclonal antibodies directed against T helper (TH) and T cytotoxic/suppressor (TCS) cells were used to identify lymphocyte subpopulations in the allograft aspirates and peripheral blood. The ratio of TH:TCS cells in both the allograft aspirates and peripheral blood decreased significantly (p less than 0.01) during the three days before rejection became clinically manifest. During rejection, the aspirate TH:TCS ratios, but not the peripheral blood TH:TCS ratios, remained depressed. Following successful treatment for rejection, the aspirate TH:TCS ratios returned to levels found in non-rejecting allografts. However, in the allografts which were lost as a result of rejection or had persistent rejection despite treatment, the aspirate TH:TCS ratios, but not peripheral blood TH:TCS ratios, remained significantly low (p less than 0.01) when compared with the successfully treated allografts. Our study indicates that TH:TCS ratios, particularly in allograft aspirates, may be of value in predicting the onset and outcome of renal allograft rejection.
为了研究肾移植患者同种异体移植物和外周血中T淋巴细胞亚群的变化，我们在移植后的第一个月内检查了24例患者的72份移植肾细针穿刺抽吸物和56份外周血样本。在排斥反应发作前、发作期间和发作后对患者进行了研究。使用针对T辅助（TH）细胞和T细胞毒性/抑制（TCS）细胞的单克隆抗体来识别移植肾穿刺抽吸物和外周血中的淋巴细胞亚群。在排斥反应临床表现出现前的三天内，移植肾穿刺抽吸物和外周血中TH:TCS细胞的比例均显著下降（p<0.01）。在排斥反应期间，穿刺抽吸物中TH:TCS比例持续降低，但外周血中TH:TCS比例未降低。成功治疗排斥反应后，穿刺抽吸物中TH:TCS比例恢复到未发生排斥反应的移植肾中的水平。然而，因排斥反应而丧失功能或尽管接受治疗仍持续发生排斥反应的移植肾，与成功治疗的移植肾相比，穿刺抽吸物中TH:TCS比例仍显著较低（p<0.01），但外周血中TH:TCS比例未降低。我们的研究表明，TH:TCS比例，尤其是移植肾穿刺抽吸物中的该比例，可能对预测肾移植排斥反应的发生和结果具有价值。
