Muraoka S, Ehman D L, Miller R G
Eur J Immunol. 1984 Nov;14(11):1010-6. doi: 10.1002/eji.1830141109.
When added to a mixed lymphocyte culture, cells in T cell colonies grown from bone marrow (BM) suppressed the development of cytotoxic activity against H-2 antigens shared by the colony cells and the stimulator cells, apparently by inactivating cytotoxic T lymphocyte precursor cells (CTLP). From the point of view of the added suppressor cells, the suppression was against self-reactive cells. The suppressor cells were resistant to gamma irradiation (1500 rds) but sensitive to UV irradiation. Inactivated CTLP separated from the suppressor cells by cell sorting could not be reactivated on being recultured with fresh stimulator cells, suggesting the suppression is irreversible. There was a critical time window, extending roughly from 20 to 40 h after culture initiation, during which the suppressor cell had to be present if CTLP were to be inactivated. During the first 20 h and after 40 h of exposure to stimulator cells, CTLP were resistant to the suppressor cell. Direct experimental evidence is presented against the possibility that the suppressor cells derived from BM colonies act by augmenting the production of Lyt-2+ suppressor cells from the responder population which then produce the suppression, or that the suppressor cells interfere with an early interaction between CTLP and stimulator cells. We conclude that the suppressor cells in T cell colonies grown from BM act directly on activated CTLP and permanently inactivate them.
当添加到混合淋巴细胞培养物中时，从骨髓（BM）生长的T细胞集落中的细胞抑制了针对集落细胞和刺激细胞共有的H-2抗原的细胞毒性活性的发展，显然是通过使细胞毒性T淋巴细胞前体细胞（CTLP）失活来实现的。从添加的抑制细胞的角度来看，这种抑制作用是针对自身反应性细胞的。抑制细胞对γ射线照射（1500拉德）具有抗性，但对紫外线照射敏感。通过细胞分选从抑制细胞中分离出的失活CTLP在用新鲜刺激细胞重新培养时不能被重新激活，这表明这种抑制作用是不可逆的。存在一个关键的时间窗口，大致从培养开始后的20至40小时，在此期间如果要使CTLP失活，抑制细胞必须存在。在暴露于刺激细胞的最初20小时内以及40小时后，CTLP对抑制细胞具有抗性。本文提供了直接的实验证据，反对来自BM集落的抑制细胞通过增加来自反应群体的Lyt-2 +抑制细胞的产生来发挥作用，然后这些细胞产生抑制作用，或者抑制细胞干扰CTLP与刺激细胞之间早期相互作用的可能性。我们得出结论，从BM生长的T细胞集落中的抑制细胞直接作用于活化的CTLP并使其永久失活。
