Kolarov J, Hatalová I
FEBS Lett. 1984 Dec 3;178(1):161-4. doi: 10.1016/0014-5793(84)81262-4.
The intracellular transport of newly synthesized beta-subunits of the F1-ATPase (beta F1) and of newly synthesized ADP/ATP carrier was followed in isolated rat hepatoma cells. As tested by rapid fractionation of [35S]methionine pulse- and pulse-chase-labeled cells and by sensitivity of labeled polypeptides to externally added protease, the import of beta F1 into mitochondria was strongly inhibited by the additional low concentrations of rhodamine 6G (R6G). In contrast, the import of the ADP/ATP carrier into mitochondria was not affected by the inhibitor. The results imply that the proteolytic processing of the precursor of beta F1 is coupled to its translocation across the mitochondrial membrane.
在分离的大鼠肝癌细胞中追踪了新合成的F1 - ATP酶β亚基（βF1）和新合成的ADP/ATP载体的细胞内运输过程。通过对[35S]甲硫氨酸脉冲标记和脉冲追踪标记细胞进行快速分级分离，并检测标记多肽对外部添加蛋白酶的敏感性，发现低浓度的罗丹明6G（R6G）能强烈抑制βF1向线粒体的导入。相比之下，ADP/ATP载体向线粒体的导入不受该抑制剂的影响。结果表明，βF1前体的蛋白水解加工与其在线粒体内膜的转运相偶联。
