Dawkins B G, Arpino P J, McLafferty F W
Biomed Mass Spectrom. 1978 Jan;5(1):1-6. doi: 10.1002/bms.1200050102.
Key steps in a proposed automated system for polypeptide sequencing utilizing a liquid chromatograph mass spectrometer computer system have been tested with mixtures containing up to six model oligopeptides. At the low nanomole level it was possible to obtain complete sequence information for all components in many, but not all, of the mixtures tried. Interpretation of the results is complicated by the presence of numerous side-products formed in the derivatization process. Minimization of such impurities will be necessary to reduce the ambiguity of the sequence information resulting from more complex mixtures, such as those expected from the degradation of larger polypeptides, and to reduce sample requirements to the subnanomole level. However, the present system appears to have unique advantages over other proposed automated methods.
利用液相色谱 - 质谱联用计算机系统进行多肽测序的拟议自动化系统中的关键步骤，已在含有多达六种模型寡肽的混合物上进行了测试。在低纳摩尔水平下，对于许多（但并非全部）所测试的混合物中的所有组分，有可能获得完整的序列信息。衍生化过程中形成的大量副产物的存在使结果的解读变得复杂。要减少来自更复杂混合物（如较大多肽降解所预期的混合物）的序列信息的模糊性，并将样品需求降低至亚纳摩尔水平，必须尽量减少此类杂质。然而，目前的系统似乎比其他拟议的自动化方法具有独特优势。
