Maack T, Marion D N, Camargo M J, Kleinert H D, Laragh J H, Vaughan E D, Atlas S A
Am J Med. 1984 Dec;77(6):1069-75. doi: 10.1016/0002-9343(84)90190-6.
Auriculin is a potent vasoactive and natriuretic peptide that was recently isolated and purified from rat atrial tissue. Since this peptide could be of great importance for renal, cardiovascular, and volume homeostasis, its functional properties have been characterized in dogs. The effects of synthetic auriculin on renal function, mean blood pressure, plasma renin activity, renin secretory rate, and plasma aldosterone levels were determined. Auriculin was administered intravenously as a prime (1.0 microgram/kg body weight) and constant infusion (0.1 microgram per minute/kg body weight for one hour) to five anesthetized dogs. In addition, two conscious dogs were used to verify some of the results obtained in anesthetized dogs. Auriculin decreased mean blood pressure from 134 +/- 5 to 122 +/- 4 mm Hg (p less than 0.05, paired t test) and increased glomerular filtration rate (25.5 +/- 2.7 to 32.4 +/- 4.1 ml per minute per kidney, p less than 0.05), diuresis (0.21 +/- 0.03 to 1.06 +/- 0.14 ml per minute per kidney, p less than 0.05), natriuresis (38 +/- 0.6 to 187 +/- 35 mueq per minute per kidney, p less than 0.05), and kaliuresis (14.8 +/- 1.6 to 35.7 +/- 6.3 mueq per minute per kidney, p less than 0.05). These effects were sustained throughout the infusion of auriculin and were entirely reversible. Renal plasma flow increased transiently for one to two minutes, and then returned to or below control levels. Urine osmolality decreased by 40 percent (p less than 0.05) whereas free water clearance remained unchanged (p less than 0.05). Auriculin reversibly decreased plasma renin activity (11.6 +/- 2.3 to 3.6 +/- 1.2 ng/ml per hour, p less than 0.05), renin secretory rate (895 +/- 313 to 255 +/- 28 ng per hour per minute, p less than 0.05), and plasma aldosterone levels (8.4 +/- 1.6 to 3.6 +/- 0.7 ng/dl, p less than 0.05), whereas plasma cortisol levels remained unchanged. These results demonstrate that auriculin has a unique combination of functional properties, increasing glomerular filtration rate, diuresis, and natriuresis, without a sustained increase in total renal blood flow, and lowering blood pressure, plasma renin levels, renin secretory rate, and plasma aldosterone levels. These properties suggest an important potential role for atrial natriuretic peptides in the regulation of renal function, extracellular volume, and blood pressure.
耳尿钠素是一种强效的血管活性和利钠肽，最近从大鼠心房组织中分离并纯化出来。由于这种肽可能对肾脏、心血管和容量稳态具有重要意义，因此已在犬类中对其功能特性进行了表征。测定了合成耳尿钠素对肾功能、平均血压、血浆肾素活性、肾素分泌率和血浆醛固酮水平的影响。将耳尿钠素以初始剂量（1.0微克/千克体重）静脉注射，并以持续输注（每分钟0.1微克/千克体重，持续一小时）的方式给予五只麻醉犬。此外，使用两只清醒犬来验证在麻醉犬中获得的一些结果。耳尿钠素使平均血压从134±5毫米汞柱降至122±4毫米汞柱（p<0.05，配对t检验），并增加了肾小球滤过率（每肾每分钟从25.5±2.7毫升增加到32.4±4.1毫升，p<0.05）、利尿（每肾每分钟从0.21±0.03毫升增加到1.06±0.14毫升，p<0.05）、尿钠排泄（每肾每分钟从38±0.6微当量增加到187±35微当量，p<0.05）和尿钾排泄（每肾每分钟从14.8±1.6微当量增加到35.7±6.3微当量，p<0.05）。这些作用在耳尿钠素输注过程中持续存在，并且完全可逆。肾血浆流量短暂增加一到两分钟，然后恢复到或低于对照水平。尿渗透压降低了40%（p<0.05），而自由水清除率保持不变（p<0.05）。耳尿钠素可逆地降低血浆肾素活性（每小时从11.6±2.3纳克/毫升降至3.6±1.2纳克/毫升，p<0.05）、肾素分泌率（每分钟每小时从895±313纳克降至255±28纳克，p<0.05）和血浆醛固酮水平（从8.4±1.6纳克/分升降至3.6±0.7纳克/分升，p<0.05），而血浆皮质醇水平保持不变。这些结果表明，耳尿钠素具有独特的功能特性组合，可增加肾小球滤过率、利尿和尿钠排泄，而不会使总肾血流量持续增加，并降低血压、血浆肾素水平、肾素分泌率和血浆醛固酮水平。这些特性表明心房利钠肽在调节肾功能、细胞外液量和血压方面具有重要的潜在作用。
