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Preservation of functional integrity during long term storage of a biological membrane.

作者信息

Mouradian R, Womersley C, Crowe L M, Crowe J H

出版信息

Biochim Biophys Acta. 1984 Dec 19;778(3):615-7. doi: 10.1016/0005-2736(84)90414-0.

DOI:10.1016/0005-2736(84)90414-0
PMID:6239654
Abstract

Sarcoplasmic reticulum vesicles freeze-dried in the presence of trehalose retain most of their original biological activity for short periods. When the dry vesicles are stored for longer periods in air, Ca2+-transport becomes uncoupled from ATPase activity within a few days. However, when they are stored under vacuum, ATPase activity, Ca2+ transport, and coupling between Ca2+ transport and ATP utilization are maintained essentially intact for at least 110 days.

摘要

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