牛心脏传导系统中ATP酶活性及肌球蛋白亚基组成的特征

Characteristics of ATPase activity and the subunit composition of myosin in the conduction system of bovine heart.

作者信息

Tamura Y, Nishimoto T, Miyakami S, Sako H, Saito K, Mori H

出版信息

FEBS Lett. 1984 Dec 10;178(2):209-12. doi: 10.1016/0014-5793(84)80602-x.

Abstract

The ATPase activity, light chains and isoenzymes of myosin from specialized myocardial tissue (the A-V node, bundle of His, and right and left bundle branches) of bovine heart were compared with those of atrial and ventricular myosins. The order of Ca2+-activated ATPase activity was atrial greater than specialized myocardial tissue greater than ventricular myosin. SDS-polyacrylamide gel electrophoresis showed that myosin from the specialized myocardial tissue contained the light chains of both atrial and ventricular myosins. On the other hand, the specialized myocardial tissue contained one V3 isomyosin and showed no difference from ventricular myocardial tissue on pyrophosphate gel.

摘要

将牛心脏特殊心肌组织（房室结、希氏束以及左右束支）中的肌球蛋白的ATP酶活性、轻链和同工酶与心房和心室肌球蛋白的这些指标进行了比较。钙离子激活的ATP酶活性顺序为：心房肌球蛋白＞特殊心肌组织＞心室肌球蛋白。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳显示，特殊心肌组织中的肌球蛋白含有心房和心室肌球蛋白的轻链。另一方面，特殊心肌组织含有一种V3同工肌球蛋白，在焦磷酸凝胶上与心室肌组织无差异。