Milewich L, Garcia R L, Gerrity L W
J Steroid Biochem. 1984 Nov;21(5):529-38. doi: 10.1016/0022-4731(84)90327-3.
The metabolism of estrone sulfate and dehydroisoandrosterone sulfate to the free, unconjugated steroids, estrone and dehydroisoandrosterone, was demonstrated in more than thirty different tissues from male and female BALB/c mice. The activity of steroid sulfatase, when expressed per mg tissue, was greatest in both the pituitary gland and the adrenal glands. The pituitary gland, however, had the lowest capacity for hydrolysis of steroid sulfates while the liver had the greatest capacity. 17 beta-Hydroxysteroid oxidoreductase activity also was demonstrated in all mouse tissues by the formation of estradiol-17 beta when using estrone sulfate as the substrate. The highest apparent activity for 17 beta-hydroxysteroid oxidoreductase was found in lung tissue, and the greatest capacity to form estradiol-17 beta from estrone sulfate was found in liver, lungs, kidneys and testes. This study demonstrates that the majority of mouse tissues have steroid sulfatase and 17 beta-hydroxysteroid oxidoreductase activities.
在雄性和雌性BALB/c小鼠的三十多种不同组织中，已证实硫酸雌酮和硫酸脱氢表雄酮可代谢为游离的、未结合的类固醇——雌酮和脱氢表雄酮。当以每毫克组织来表示时，类固醇硫酸酯酶的活性在垂体和肾上腺中最高。然而，垂体水解类固醇硫酸盐的能力最低，而肝脏的能力最强。当以硫酸雌酮为底物时，通过生成雌二醇-17β，在所有小鼠组织中也证实了17β-羟基类固醇氧化还原酶活性。在肺组织中发现17β-羟基类固醇氧化还原酶的表观活性最高，而从硫酸雌酮形成雌二醇-17β的能力在肝脏、肺、肾脏和睾丸中最强。这项研究表明，大多数小鼠组织具有类固醇硫酸酯酶和17β-羟基类固醇氧化还原酶活性。
